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Protocol

Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions

  • Amanda Lipsitt 1
  • Nicole R. Hensch 2
  • Rodrigo Moreno-Campos 2
  • Kunal Baxi 2
  • Jiangfei Chen 2
  • Anil K. Challa 2
  • Eleanor Y. Chen 3
  • Myron S. Ignatius 2
  • 1 - Department of Pediatrics - Division of Hematology Oncology - UT Health Sciences Center - San Antonio - Texas USA
  • 2 - Department of Molecular Medicine - Greehey Children's Cancer Research Institute (GCCRI) - UT Health Sciences Center - San Antonio - Texas USA
  • 3 - Department of Laboratory Medicine and Pathology - University of Washington - Seattle - Washington USA

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.


 


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