Protocols and Applications
Protocol
Is Your AAV Payload Impacting Stability? Find out with Aura GT
Adeno-associated viruses (AAVs) have transformed gene therapies by enabling the targeted delivery of therapeutic genetic payloads. However, the amount of AAV material available is consistently in short supply due to its inherently expensive, time-consuming, and laborious production process. This ham... More
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
Preparing a Brain-Mimetic 3D Matrix for Culturing Human Pluripotent Stem Cells
This is a protocol for culturing human pluripotent stem cells (hPSCs) using a 3D matrix. It provides instructions for preparing a brain-mimetic 3D scaffold that can support neuronal growth and for culturing hPSCs. This injectable scaffold can be potentially employed in the field of regenerative medi... More
Application
Knock-out cell lines and their use in signaling pathways
Knock-out (KO) cell lines are powerful tools to study disease mechanisms, such as the effect of gene disruption on downstream pathways. In this application note, we highlight the effectiveness of Abcam KO cell lines that can be used to investigate signaling pathways, in this case, the TRAIL pathway.... More
Application
Recombinant RabMAb®: Reliable antibodies for reproducible results
Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More
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