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Protocols and Applications

Application

Technical tips for ELISA and multiplex immunoassay development

Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More


Protocol

Filter Capture, Extraction, and Sequencing of Coastal Metagenomes

In order to analyze the metagenomes of coastal microbial communities, we have standardized a protocol to collect, extract, and sequence near-shore microbiome samples. This procedure involves on-site filter capture of microbiomes from coastal water, DNA extraction via ceramic bead processing and sili... More


Application

Benchling for RNA Therapeutics R&D

Because RNA therapeutics can target conditions that small molecules and proteins cannot address, researchers are now on the verge of treating previously “undruggable” diseases. From the approval of the first antisense oligonucleotide, aptamer, and siRNA, to the now famous launch of mRNA-based COVID-... More


Application

Benchling for Cell Therapy R&D

Cell therapies such as stem cells, CAR T cells, and TCR have been of significant interest in research over the past few decades. The FDA approval of CAR T therapies has led to renewed interest in the field. As companies rush to bring more novel cell therapies to the market, they face several R&D com... More


Application

Benchling for Hybridoma Technology

Hybridoma technology is a well-established method for generating monoclonal antibodies (mAB), but various scientific and organizational challenges persist. Laborious and resource-intensive processes, low titers, and complex assays mean that scientists have to streamline every step, simplify handoffs... More


Application

Benchling for Protein and Peptide Therapeutics R&D

Proteins and peptides make up a wide variety of therapeutic molecules ranging from enzymes to cytokines. These therapeutics have several biological functions and interact with various biological pathways. New classes of proteins and peptides continue to be of significant research interest, but there... More


Application

Benchling for CRISPR Screening

CRISPR screening is a revolutionary methodology to identify genes of interest underlying a specific phenotype. CRISPR can be scaled up for screening applications because of the versatility of CRISPR/Cas9 in disrupting target DNA and the ease of gRNA/plasmid generation. However, large-scale use of th... More


Application

Benchling for Industrial Biotech R&D

Industrial biotechnology is transforming traditional manufacturing technologies by providing more eco-friendly approaches like bioprocessing and biomanufacturing. However, there are several hurdles for industrial biotech companies seeking to develop next-gen strains, novel processes, and new applica... More


Protocol

Radiolabelling Nucleic Acids with [γ-32P] ATP for Analytical Assays

Nucleic acids are readily modified with tags that enable detection, purification, or tracking their interactions with other molecules. One of the standard labels used to generate nucleic acid probes includes radioactive phosphates because they allow for sensitive and rapid detection. This protocol d... More


Protocol

Generating and maintaining uniform 3D spheroids in long term cultures

This instruction explains the basic operating procedure for generating and maintaining longterm three-dimensional spheroid cultures on PrimeSurface® 96 Slit-well plates. Instructions recommended here are the best operating protocols but depending upon your cell line and assay, some optimizations may... More