Protocols and Applications
Protocol
Isolation of Mononuclear Cell Fractions Using Your Sepax™ System
The purpose of this protocol is to provide instructions on how to isolate mononuclear cell fractions of apheresis product, bone marrow, or umbilical cord blood using the Sepax™ system with initial volumes of 30 to 120 mL and selectable final volumes of 8-20 mL or 45 mL. This protocol applies to anyo... More
Protocol
Cell culture CO2 incubator decontamination process using hydrogen peroxide vapor for highly regulated and general cell culture protocols
The value of the laboratory cell culture incubator used in highly regulated research and clinical protocols is directly related to the proportion of incubator uptime vs. downtime in applications where frequent interior chamber decontamination is required or desired. The need for interior decontamina... More
Protocol
Sorting of water-in-oil emulsions with bacteria using a fluorescent nucleic acid probe
To isolate water-in-oil emulsion droplets containing bacteria from those that do not contain bacteriawe have developed a simple and reliable selective sorting method that allows bacteria-containing droplets to be isolated on our microfluidic chip cell sorter, On-chip Sort. Bacteria-containing drople... More
Protocol
Removal of rare undifferentiated induced pluripotent stem cells (iPSCs) by multi-step negative sorting
In the field of regenerative medicine involving ES or iPS cells, differentiated cells are often cultured to tissues and implanted to patients. However, there is an unneglectable problem that some remaining undifferentiated cells in the implanted tissue may cause tumors. Therefore, a complete removal... More
Application
Benchling & Beam Therapeutics: A Two-Pronged Approach to Automation for Gene Therapy Research
Beam Therapeutics is developing a new class of genetic medicines using novel base editing technology that enables them to edit precise sets of base pairs without introducing a double-stranded break in the DNA. Beam needs to evaluate thousands of biological samples each week, but their digital infras... More
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