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Protocols and Applications

Protocol

Radiolabelling Nucleic Acids with [γ-32P] ATP for Analytical Assays

Nucleic acids are readily modified with tags that enable detection, purification, or tracking their interactions with other molecules. One of the standard labels used to generate nucleic acid probes includes radioactive phosphates because they allow for sensitive and rapid detection. This protocol d... More


Protocol

Generating and maintaining uniform 3D spheroids in long term cultures

This instruction explains the basic operating procedure for generating and maintaining longterm three-dimensional spheroid cultures on PrimeSurface® 96 Slit-well plates. Instructions recommended here are the best operating protocols but depending upon your cell line and assay, some optimizations may... More


Protocol

Noninvasive 3D imaging Using Optical Coherence Tomography

This protocol describes a novel OCT-based technology for imaging and analysis of 3D structures, such as, spheroid /organoids, tissues for growth morphological evaluation, quantification of internal cavities, drug sensitivity testing to capture the events leading to tumor cell death, and assessing e... More


Protocol

Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates

Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More


Protocol

Performing a manual run on your Xuri™ Cell Expansion System W25

The purpose of this protocol is to provide instructions on how to perform a manual run on the Xuri™ Cell Expansion System W25 using UNICORN™ software.This protocol applies to anyone performing a manual run on the Xuri™ Cell Expansion System W25.... More