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Protocol Primers

Technical briefs on current and emerging biotech protocols.

© Doug Auld, PhD/Novartis Institutes for BioMedical Research

Primer

Is Your Flow Cytometer Reliable?

Aug 31, 2021

Flow cytometry provides a useful method by which scientists can measure and analyse different properties of individual cells, including size, internal complexity (granularity) and the presence of cell surface and intracellular markers using fluorescence. Flow can help identify individual cell subpop... More


© chrissmith12/Pixabay

Primer

Unwanted Fungi: Detecting Microbial Cell Culture Contamination

Aug 31, 2021

Coming into the lab and spotting an obvious fungal growth, or the tell-tale signs of cloudy media, can be a stressful experience for researchers. When carrying out cell culture in the lab, contamination from microbial agents such as bacteria and fungus can occur and have disastrous effects on ongoin... More


© Monty Rakusen / Cultura / Getty Images

Primer

Source and Elimination of Common Mass Spectrometry Contaminants

Aug 31, 2021

Sensitivity is a key attribute of mass spectrometers to allow quantification of minute amounts of analytes. Yet this sensitivity can be a conundrum. Myriad sources can contribute contaminants that can impact background and confound interpretation of results. An increased background signal can make i... More