Protocol Primers
Technical briefs on current and emerging biotech protocols.
Primer
Challenges of Organoid Culture
Dec 20, 2021
Complex patient models, including organoids and organ-on-a-chip devices, have been developing tremendously fast over the last decade.1 Organoids are 3D multicellular in vitro tissue constructs that functionally and genetically mimic portions of in vivo organs. Properly supplemented media allow the c... More
Primer
Separating Blood into Cellular and Non-Cellular Components
Nov 30, 2021
A key diagnostic step in disease detection is analyzing blood components. Accurate separation of blood cells from plasma, which holds proteins and other constituents, is crucial in many assays.First introduced in the late 1980s, lateral flow devices, also referred to as lateral flow immunoassays, ar... More
Primer
Saliva, Nasal Swabs, or Blood: Best Samples for Pathogen Detection
Nov 22, 2021
Most immunodiagnostic protocols and commercially available kits require serum, plasma, or blood samples. Collecting such samples requires the skills of a phlebotomist. For pathogen detection, particularly in largescale field studies, the collection of noninvasive samples such asnasal swabs, tears, o... More
Primer
Qualitative, Semi-quantitative, and Quantitative Immunodiagnostics for COVID-19
Nov 10, 2021
While qualitative reverse transcription polymerase chain reactions (RT-PCR) are used to diagnose COVID-19, serologic tests for antibodies specific to the SARS-CoV-2 virus are used to detect past infection. Most serologic assays are qualitative and semi-quantitative but quantitative serologic assays ... More
Primer
Benefits of Using an Electronic Laboratory Notebook
Oct 30, 2021
Electronic laboratory notebooks (ELNs) were developed to keep track of experiments and data so that scientists could review their work in a single place and easily reproduce experiments. Many ELNs started out as electronic versions of paper lab notebooks. Now, the most advanced ELNs offer an extensi... More
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Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
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An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish -
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A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration -
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Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function -
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Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
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Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More