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Protocol Primers

Technical briefs on current and emerging biotech protocols.

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Primer

Assessing qRT-PCR COVID-19 Testing Results: What Can Go Wrong?

Sep 20, 2021

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes--an RNA-dependent DNA polymerase, also known as a reverse transcriptase, to copy the SARS-CoV-2 RNA into complement... More


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Primer

Assessing Protein Purity: Many Tests, Many Assays

Aug 31, 2021

Proteins provide structure to cells, catalyse enzymatic reactions, transport cargo, replicate DNA, metabolise nutrients among an array of other functions. Composed of amino acids and generally featuring a three-dimensional structure, proteins form key therapeutic and diagnostic tools as antibodies o... More


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Primer

Two Critical Steps to Success in Multicolor Flow Cytometry

Aug 31, 2021

Flow cytometry is a powerful tool to analyze and characterize cells based on their protein expression, most often those expressed on the cell surface. Multicolor flow cytometry uses multiple fluorochromes as primary or secondary antibody conjugates to label cells. The capacity for modern flow cytome... More


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Primer

Expressing, Extracting and Purifying Membrane Proteins

Aug 31, 2021

Membrane proteins are key biological players involved in myriad essential cellular processes from molecular transport, cellular communication, and signal transduction to maintaining structures within cells and tissues. Despite their importance, accounting for 25% of all proteins in the human genome ... More


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Primer

Considerations when Selecting a Matrix for MALDI

Aug 31, 2021

One of the most widely utilized techniques in mass spectrometry imaging (MSI) is label-free MALDI (Matrix-Assisted Laser Desorption Ionization), which can be coupled to a variety of mass analyzers to create brilliant molecular maps. Application targets include lipids, proteins, peptides, drugs, and ... More