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GEN Protocols


Mapping Tissue Carbohydrates Using Mass Spectrometry

Date: March 21, 2022, 11:00 am - 12:00 pm ET

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This mixer will be relevant for all interested in methods used in glycoproteomics research that aims to understand the pivotal role of carbohydrate motifs that modify the proteome. 

Glycoproteomics, a dynamic area of frontier research, provides a systems biology approach to understanding the biological attributes and functions of glycosylation in biological processes as diverse as immune response, development, differentiation, adhesion and host-pathogen interactions. Over half of all human proteins bear N-linked glycosylations with vital roles in protein folding, molecular trafficking, signal transduction, cell-cell interactions, and other processes. Changes in glycosylation have been found in diseases ranging from cancer and cystic fibrosis to Parkinson disease and arthritis. In addition to deciphering disease etiology, glycoproteins are ideal biomarkers as they are assessable in minimally invasive serum samples. Despite its importance in biology, glycoproteomic analyses remain analytically challenging, partly because glycosylation mechanisms are complex and heterogenous. Imaging mass spectrometry (IMS) is a powerful tool to detect changes in N-linked glycosylation to bolster histopathologic insights from standard formalin-fixed paraffin embedded tissue samples.

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Richard Drake, Ph.D. is a Professor and SmartState Endowed Chair in Proteomics at Medical University of South Carolina, and co-founder/acting CEO of GlycoPath INC. His current research interests are centered on developing MALDI imaging mass spectrometry workflows for the analysis of N-glycans and other glycomic structures in cancer tissues and liquid biopsy samples.