Translational Medicine
Translational Medicine
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
Preparing a Brain-Mimetic 3D Matrix for Culturing Human Pluripotent Stem Cells
This is a protocol for culturing human pluripotent stem cells (hPSCs) using a 3D matrix. It provides instructions for preparing a brain-mimetic 3D scaffold that can support neuronal growth and for culturing hPSCs. This injectable scaffold can be potentially employed in the field of regenerative medi... More
Protocol
How to run cryopreservation protocols using a VIA Freeze™ system
The purpose of this protocol is to give the reader a comprehensive understanding of how to set up and operate the Cytiva VIA Freeze™ Uno, VIA Freeze™ Duo, and VIA Freeze™ Quad units.This protocol applies to anyone performing a procedure with the VIA Freeze™ units. Once training on this document is c... More
Protocol
Isolation of Mononuclear Cell Fractions Using Your Sepax™ System
The purpose of this protocol is to provide instructions on how to isolate mononuclear cell fractions of apheresis product, bone marrow, or umbilical cord blood using the Sepax™ system with initial volumes of 30 to 120 mL and selectable final volumes of 8-20 mL or 45 mL. This protocol applies to anyo... More
Protocol
Rapid metabolic phenotyping in SARS-CoV-2 patients using the impact II QTOF MS system
The impact II QTOF system meets the critical elements of sensitivity, selectivity, accuracy, and precision for targeted metabolite analysis. The continuous collection of both MS and MS/MS data provides an exponentially deeper and broader pool of data valuable for subsequent retrospective discovery o... More
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