Translational Medicine
Translational Medicine
Protocol
Removal of rare undifferentiated induced pluripotent stem cells (iPSCs) by multi-step negative sorting
In the field of regenerative medicine involving ES or iPS cells, differentiated cells are often cultured to tissues and implanted to patients. However, there is an unneglectable problem that some remaining undifferentiated cells in the implanted tissue may cause tumors. Therefore, a complete removal... More
Protocol
A Protocol for Qualifying DNA Variants Associated with Complex Diseases like Ehlers-Danlos syndrome
A protocol for DNA variant qualification adds medical perspective to consensus grading based on impact of the DNA change on its encoded RNA/protein and its prevalence in normal people versus those with the implicated disease. This clinically familiar 1-4+ qualification can encourage physician accept... More
Protocol
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions
Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, e... More
Protocol
Predictive Low-Glucose Suspend Necessitates Less Carbohydrate Supplementation to Rescue Hypoglycemia: Need to Revisit Current Hypoglycemia Treatment Guidelines
Current guidelines recommend 15–20 g of carbohydrate (CHO) for treatment of mild to moderate hypoglycemia. However, these guidelines do not account for reduced insulin during suspensions with predictive low-glucose suspend (PLGS). We assessed insulin suspensions, hypoglycemic events, and CHO treatme... More
Protocol
Insulin Metrics: Need for Development of Consensus Standards for Reporting of Insulin Dosing Data
The advent of connected insulin pens will generate an avalanche of digital insulin data, especially in the context of prandial- and multiple daily injection-insulin regimens. There is a need for the diabetes community to develop standards for such data, analogous to what has been achieved using the ... More
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Protocol
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
Protocol
An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish -
Protocol
A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration -
Protocol
Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function -
Protocol
Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
Protocol
Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More