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GEN Protocols

Translational Medicine

Translational Medicine

Primer

Tips for Increasing Transfection Efficiency

Chemical transfection, which relies on the formation of a condensed complex of positively chargedliposomal or non-liposomal reagents and negatively charged nucleic acids,is the most common method for delivering foreign genetic material into cultured cells. Complexes are attracted to the negatively c... More


Primer

Methods to Improve Efficiency of Antimicrobial Susceptibility Testing: Microfluidic Impedance Cytometry

The global issue of antimicrobial resistance has been called a “slow motion catastrophe” resulting from the excessive and often inappropriate use of antibiotics to fight bacterial infections.1 A long-standing unmet need is the availability of a quick and easy clinical test to support a more evidence... More


Primer

Bio-Electrospraying and Cell Electrospinning: A Sea of Applications

Electrospraying and electrospinning have been used for a wide range of applications ranging from coatings, propulsion, and pesticide sprays to scaffolding a wide range of advanced materials for applications in filtration and tissue engineering. Although electrospun scaffolds have been explored as ti... More


Primer

AI-Powered Phenotypic Profiling Revolutionizes Drug Discovery

Today, nine out of ten drugs fail in clinical trials. Furthermore, it takes over a decade and an average cost of $2 billion to develop and approve each medicine.1 An important underlying reason is the gap that exists between cell-based in vitro research and clinical research—often referred to as the... More


Primer

Rapid Detection of Diverse Infectious Agents in Skin Interstitial Fluid

A rapid and reliable skin-patch test1 can now detect the COVID-19 virus, and potentially other infectious agents in under three minutes, without the need to draw blood. This convenience overcomes a current challenge in identifying infected individuals who are averse to blood tests and could help res... More


Primer

Cloning Mice From Freeze Dried Somatic Cells

As species continue to face massive extinction, preserving genetic material through judicious biobanking to enable cloning is key to promoting the survival of species and maintaining biodiversity.Embryos and germ cells are commonly stored at ultra-low temperatures in liquid nitrogen. But storing rep... More


Primer

Behavior of Metal Bearing Nanomaterials in Biosystems

Nanomaterials are being engineered to facilitate a range of applications in diagnosing diseases and delivering drugs. The widespread adoption of nanotechnological applications in medicine rests on the ability to safely target nanomaterials and understand how they behave in biological tissue environs... More


Primer

Nanotip Stabilization Enables Prolonged Nanoscale Imaging of Large Samples

An innovation that stabilizes the nanotip used in tip-enhanced Raman spectroscopy (TERS) prolongs the imaging time window well beyond the current 30 minute limit. Scientists have demonstrated that the enhanced system is capable of imaging nanoscale defects over six hours in a micrometer-sized, two-d... More


Protocol

A Protocol for Qualifying DNA Variants Associated with Complex Diseases like Ehlers-Danlos syndrome

A protocol for DNA variant qualification adds medical perspective to consensus grading based on impact of the DNA change on its encoded RNA/protein and its prevalence in normal people versus those with the implicated disease. This clinically familiar 1-4+ qualification can encourage physician accept... More


Protocol

Investigating the increased lifespan in C. elegans daf-2 mutants by 4D-Lipidomics™

The small nematode Caenorhabditis elegans is one of the premier biomedical model organisms and employed in many aspects of basic and applied science.... More


Protocol

A Protocol for Qualifying DNA Variants Associated with Complex Diseases like Ehlers-Danlos syndrome

A protocol for DNA variant qualification adds medical perspective to consensus grading based on impact of the DNA change on its encoded RNA/protein and its prevalence in normal people versus those with the implicated disease. This clinically familiar 1-4+ qualification can encourage physician accept... More


Protocol

Investigating the increased lifespan in C. elegans daf-2 mutants by 4D-Lipidomics™

The small nematode Caenorhabditis elegans is one of the premier biomedical model organisms and employed in many aspects of basic and applied science.... More


Protocol

4D-Lipidomics™ workflow for increased throughput

Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and ... More


Protocol

Rapid metabolic phenotyping in SARS-CoV-2 patients using the impact II QTOF MS system

The impact II QTOF system meets the critical elements of sensitivity, selectivity, accuracy, and precision for targeted metabolite analysis. The continuous collection of both MS and MS/MS data provides an exponentially deeper and broader pool of data valuable for subsequent retrospective discovery o... More


Protocol

Removal of rare undifferentiated induced pluripotent stem cells (iPSCs) by multi-step negative sorting

In the field of regenerative medicine involving ES or iPS cells, differentiated cells are often cultured to tissues and implanted to patients. However, there is an unneglectable problem that some remaining undifferentiated cells in the implanted tissue may cause tumors. Therefore, a complete removal... More


Protocol

Sorting of water-in-oil emulsions with bacteria using a fluorescent nucleic acid probe

To isolate water-in-oil emulsion droplets containing bacteria from those that do not contain bacteriawe have developed a simple and reliable selective sorting method that allows bacteria-containing droplets to be isolated on our microfluidic chip cell sorter, On-chip Sort. Bacteria-containing drople... More


Protocol

Cell culture CO2 incubator decontamination process using hydrogen peroxide vapor for highly regulated and general cell culture protocols

The value of the laboratory cell culture incubator used in highly regulated research and clinical protocols is directly related to the proportion of incubator uptime vs. downtime in applications where frequent interior chamber decontamination is required or desired. The need for interior decontamina... More


Protocol

Isolation of Mononuclear Cell Fractions Using Your Sepax™ System

The purpose of this protocol is to provide instructions on how to isolate mononuclear cell fractions of apheresis product, bone marrow, or umbilical cord blood using the Sepax™ system with initial volumes of 30 to 120 mL and selectable final volumes of 8-20 mL or 45 mL. This protocol applies to anyo... More


Protocol

How to run cryopreservation protocols using a VIA Freeze™ system

The purpose of this protocol is to give the reader a comprehensive understanding of how to set up and operate the Cytiva VIA Freeze™ Uno, VIA Freeze™ Duo, and VIA Freeze™ Quad units.This protocol applies to anyone performing a procedure with the VIA Freeze™ units. Once training on this document is c... More


Protocol

Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates

Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More


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Primer

Cloning Mice From Freeze Dried Somatic Cells

As species continue to face massive extinction, preserving genetic material through judicious biobanking to enable cloning is key to promoting the survival of species and maintaining biodiversity.Embryos and germ cells are commonly stored at ultra-low temperatures in liquid nitrogen. But storing rep... More


Primer

Behavior of Metal Bearing Nanomaterials in Biosystems

Nanomaterials are being engineered to facilitate a range of applications in diagnosing diseases and delivering drugs. The widespread adoption of nanotechnological applications in medicine rests on the ability to safely target nanomaterials and understand how they behave in biological tissue environs... More


Primer

Nanotip Stabilization Enables Prolonged Nanoscale Imaging of Large Samples

An innovation that stabilizes the nanotip used in tip-enhanced Raman spectroscopy (TERS) prolongs the imaging time window well beyond the current 30 minute limit. Scientists have demonstrated that the enhanced system is capable of imaging nanoscale defects over six hours in a micrometer-sized, two-d... More


Mixer

Organoids for Airway and Alveolar Disease Modeling

In this Mixer, we discussed the development of respiratory airway and alveolar organoids derived from human iPSCs and how these are used in modeling respiratory diseases such as cystic fibrosis. Airway organoid models can be used for testing the therapeutic efficacy of candidate drugs for respirator... More


Mixer

Soft Skills Needed in the Biotech Workforce

Being a professional in a successful life science company--be it a CRO, a CDMO, a startup, a mid- to medium-sized biotech firm, or an established company with a track record of popular products--requires much more than academic credentials, research expertise and technical knowhow. Soft skills are t... More


Video

GEN Protocols Expert Exchanges: Tools and Techniques to Develop Therapies for Neurological Disorders

The human brain is an exquisitely complex organ, consisting of nearly one hundred billion neurons, an equivalent number of non-neuronal cells and trillions of synapses. Understanding the factors that play a role in the development of the structure and function of the brain is an essential step to de... More


Video

GEN Protocols Expert Exchanges: Patient-Derived Pancreatic Cancer Organoids Predict Response to Therapy

Pancreatic cancer accounts for three percent of all cancers in the United States and is responsible for seven percent of all cancer deaths. Miniature organoids cloned from pancreatic tumors in patients are being grown in laboratories to conduct assays that can predict the effectiveness of chemother... More


Video

GEN Protocols Expert Exchanges: Critical ALS Biomarkers of Neuroinflammation & Oxidative Stress

Biomarkers are essential tools for the development of therapeutics, clinical trial design and patient stratification. Biomarkers also help evaluate the efficacy of therapeutic intervention on disease progression or relapse. Yet, biomarker driven patient stratification, a hallmark in oncology trials,... More


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