Translational Medicine
Translational Medicine
Protocol
Short Communication: Ischemic Stroke Subtypes Among Thai HIV-Infected Patients: A 12-Year Retrospective Study
Traditional vascular risk factors and HIV-related factors can contribute to higher prevalence of ischemic stroke (IS) among HIV-infected patients. Understanding the underlying mechanism of IS could lead to proper management and prognosis. We aimed to determine the prevalence of IS subtypes among HIV... More
Protocol
Generating and maintaining uniform 3D spheroids in long term cultures
This instruction explains the basic operating procedure for generating and maintaining longterm three-dimensional spheroid cultures on PrimeSurface® 96 Slit-well plates. Instructions recommended here are the best operating protocols but depending upon your cell line and assay, some optimizations may... More
Protocol
Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates
Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More
Protocol
Cell culture CO2 incubator decontamination process using hydrogen peroxide vapor for highly regulated and general cell culture protocols
The value of the laboratory cell culture incubator used in highly regulated research and clinical protocols is directly related to the proportion of incubator uptime vs. downtime in applications where frequent interior chamber decontamination is required or desired. The need for interior decontamina... More
Protocol
Sorting of water-in-oil emulsions with bacteria using a fluorescent nucleic acid probe
To isolate water-in-oil emulsion droplets containing bacteria from those that do not contain bacteriawe have developed a simple and reliable selective sorting method that allows bacteria-containing droplets to be isolated on our microfluidic chip cell sorter, On-chip Sort. Bacteria-containing drople... More
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Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More