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GEN Protocols

OMICS

OMICS

Application

Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology

Precedent Protocols and Processes ensure that enrichment, stimulation and labeling will result in maximizing the correlative data achieved, while minimizing non-specific impact on the cells themselves. Highlights: • Published enrichment, stimulation, and staining protocols throughout immunotherapy ... More


Protocol

IsoCode Single-Cell Innate Immune: Human CD34+ Myeloid Stem Cell Protocol

A. Overview1. Overview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.Day 2: Enrichment and Stimulation of CD34+ cells for 24 hours.Day 3: Staining and Loading of CD34+ cells onto IsoCode chip.NOTE: This protocol outlines the standard method for thawi... More


Protocol

IsoCode Single-Cell Innate Immune: Human Monocyte Protocol Using Membrane Stain

A. OverviewOverview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in complete RPMI media.Day 2: Enrichment and Stimulation of Monocytes for 24 hours.Day 3: Staining and Loading of Monocytes onto IsoCode Chip.... More


Protocol

IsoCode Single-Cell Adaptive Immune: TCR-T Protocol

A. Overview of ProtocolDay 1: Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2. Cryopreserved target cell lines are thawed, cultured and passaged several days prior to beginning the experiment.Day 2: Enrichment, Staining, and Antigen Stimulation of CD4+ and/or CD8+... More


Protocol

Single-Cell Intracellular Proteome: HeLa Cell Protocol

Overview of Protocol:Day 1-3: Cryopreserved HeLa cells are thawed and cultured for 72 hours.Day 4: Staining and stimulating of HeLa cells followed by immediate loading onto IsoCode Chip... More


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