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GEN Protocols

OMICS

OMICS

Protocol

Ultra-high sensitivity proteomics on the timsTOF SCP

The timsTOF Pro platform, introduced in 2017, already featured a sensitivity boost from the Parallel Accumulation SErial Fragmentation (PASEF) technology, which provides time and space focusing of the ions in the Trapped Ion Mobility Spectrometry (TIMS) tunnel. The timsTOF SCP platform further enhan... More


Protocol

Rapid and sensitive label-free proteome quantification on the timsTOF Pro 2

Shotgun proteomics capacity to deliver in-depth information on the identification, the relative amount, and the modification patterns of ever larger fraction of the proteins present in a sample has made it an essential approach in most biological research areas. The recent introduction of Parallel A... More


Protocol

MALDI Guided SpatialOMx uncovers proteomic profiles in tumor subpopulations of breast cancer

The timsTOF fleX system bridges a current gap by providing MALDI Imaging and in-depth proteomics analysis in just one instrument. The instrument offers all benefits of a timsTOF Pro for timeefficient and sensitive proteomics, combined with a high-resolution MALDI source and stage. Using PASEF techno... More


Protocol

Sorting of water-in-oil emulsions with bacteria using a fluorescent nucleic acid probe

To isolate water-in-oil emulsion droplets containing bacteria from those that do not contain bacteriawe have developed a simple and reliable selective sorting method that allows bacteria-containing droplets to be isolated on our microfluidic chip cell sorter, On-chip Sort. Bacteria-containing drople... More


Protocol

Multiplexed, trackable CRISPR-based genome engineering for optimization of heterologous protein expression and activity in E. coli

This work demonstrates the use of the Onyx platform for interrogation and optimization of heterologous proteins in E. coli. Compared to traditional methods for protein engineering, such as random mutagenesis or targeted site-directed mutagenesis approaches, the Onyx technology dramatically increases... More


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