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GEN Protocols

OMICS

Protocol

Incorporating CCS values to enable 4-dimensional annotation of metabolic features

Reliable annotation of metabolites for LC-MS/MS based data requires the adept combination of many parameters such as retention time and accurate mass. To extend our parameter portfolio, we analyzed the high reproducible measured CCS values using trapped ion mobility spectrometry (TIMS) to provide ad... More


Protocol

prm-PASEF®: enabling high-throughput, high sensitivity targeted proteomics

In comparison with standard selected and parallel reaction monitoring (SRM and PRM), the prm-PASEF increases the number of peptides that can be targeted in a single acquisition method, without compromising the selectivity or the sensitivity. Using a prototype acquisition software, we targeted 201 is... More


Protocol

MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells

Probing the metabolic state of living microbial cells via their secreted metabolic products is highly challenging as it requires measures to maintain the cellular integrity and to collect the analytes. This application note demonstrates how direct infusion magnetic resonance mass spectrometry (DI-MR... More


Protocol

Ultra-high sensitivity proteomics on the timsTOF SCP

The timsTOF Pro platform, introduced in 2017, already featured a sensitivity boost from the Parallel Accumulation SErial Fragmentation (PASEF) technology, which provides time and space focusing of the ions in the Trapped Ion Mobility Spectrometry (TIMS) tunnel. The timsTOF SCP platform further enhan... More


Protocol

Rapid and sensitive label-free proteome quantification on the timsTOF Pro 2

Shotgun proteomics capacity to deliver in-depth information on the identification, the relative amount, and the modification patterns of ever larger fraction of the proteins present in a sample has made it an essential approach in most biological research areas. The recent introduction of Parallel A... More


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