OMICS
OMICS
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
Filter Capture, Extraction, and Sequencing of Coastal Metagenomes
In order to analyze the metagenomes of coastal microbial communities, we have standardized a protocol to collect, extract, and sequence near-shore microbiome samples. This procedure involves on-site filter capture of microbiomes from coastal water, DNA extraction via ceramic bead processing and sili... More
Protocol
Rapid metabolic phenotyping in SARS-CoV-2 patients using the impact II QTOF MS system
The impact II QTOF system meets the critical elements of sensitivity, selectivity, accuracy, and precision for targeted metabolite analysis. The continuous collection of both MS and MS/MS data provides an exponentially deeper and broader pool of data valuable for subsequent retrospective discovery o... More
Protocol
4D-Lipidomics™ workflow for increased throughput
Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and ... More
Protocol
Investigating the increased lifespan in C. elegans daf-2 mutants by 4D-Lipidomics™
The small nematode Caenorhabditis elegans is one of the premier biomedical model organisms and employed in many aspects of basic and applied science.... More
Protocol
Achieving Reproducible and Automated Region-Specific Lipid Annotations using CCS-aware SpatialOMx®
The CCS-aware SpatialOMx® workflow opens new dimensions by combining the molecular and spatial information measured by MALDI-TIMS Imaging with highly confident 4D-Omics annotations. MetaboScape® 2021b and SCiLS™ Lab 2021a provide the interface to match data from both ionization techniques and enable... More
Protocol
How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data
Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during product development. Typically, this analysis is conducted after enzymatic release and tagging of the glycans followed by fluorescence and MS detection. However, that approach lose... More
Protocol
High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients
In this application note, we demonstrate the benefits of dia-PASEF technology on the timsTOF Pro platform coupled to an Evosep One for high-throughput in-depth proteome analysis of up to 300 samples per day (SPD). We quantify about 5200 protein groups in only 21-minutes run time. In 4.8-minutes ultr... More
Protocol
Elucidation of metabolic changes in HFD-ApoE–/– model by SP6 peptide: A flow injection analysis magnetic resonance mass spectrometry (FIA-MRMS) study
The employment of natural peptides in the treatment of cardiovascular diseases is an attractive option to pharmacological therapy. In this study, a novel antihypertensive peptide from Spirulina Platensis (SP6) was employed to evaluate its potential anti-atherosclerotic effect in high fat diet ApoE–/... More
Protocol
Magnetic Resonance Mass Spectrometry (MRMS) discriminates yeast mutants through metabolomics
A eukaryote model of Saccharomyces cerevisiae was used to study the methylglyoxal pathway by Magnetic resonance mass spectrometry (MRMS). It was discovered that glutathione plays a major role in driving metabolomic differences between different strains. It is expected that metabolomics-based discrim... More
The Latest
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Application
A Fast and High Precision Influenza Vaccine Potency Assay -
Application
Strategies for the Development of a High Throughput Octet® Bio-Layer Interferometry Method to Measure Pharmacokinetics of Monoclonal Antibodies in Preclinical Animal Models -
Application
Effective Dead Cell Depletion using Microbubbles Delivers High Yield -
Protocol
Preclinical Optimization and Safety Studies of a New Lentiviral Gene Therapy for p47phox-Deficient Chronic Granulomatous Disease -
Protocol
High-Throughput In Vitro, Ex Vivo, and In Vivo Screen of Adeno-Associated Virus Vectors Based on Physical and Functional Transduction -
Protocol
Effective Targeting of Multiple B-Cell Maturation Antigen–Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T Cells - More