OMICS
OMICS
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
Filter Capture, Extraction, and Sequencing of Coastal Metagenomes
In order to analyze the metagenomes of coastal microbial communities, we have standardized a protocol to collect, extract, and sequence near-shore microbiome samples. This procedure involves on-site filter capture of microbiomes from coastal water, DNA extraction via ceramic bead processing and sili... More
Protocol
Rapid metabolic phenotyping in SARS-CoV-2 patients using the impact II QTOF MS system
The impact II QTOF system meets the critical elements of sensitivity, selectivity, accuracy, and precision for targeted metabolite analysis. The continuous collection of both MS and MS/MS data provides an exponentially deeper and broader pool of data valuable for subsequent retrospective discovery o... More
Protocol
4D-Lipidomics™ workflow for increased throughput
Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and ... More
Protocol
Investigating the increased lifespan in C. elegans daf-2 mutants by 4D-Lipidomics™
The small nematode Caenorhabditis elegans is one of the premier biomedical model organisms and employed in many aspects of basic and applied science.... More
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Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
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An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish -
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A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration -
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Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function -
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Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
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Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More