Drug Discovery
Drug Discovery
Protocol
Single-Cell Intracellular Proteome: HeLa Cell Protocol
Overview of Protocol:Day 1-3: Cryopreserved HeLa cells are thawed and cultured for 72 hours.Day 4: Staining and stimulating of HeLa cells followed by immediate loading onto IsoCode Chip... More
Protocol
IsoCode Single-Cell Adaptive Immune: Human Bispecific Protocol
Overview of ProtocolDay 1: Cryopreserved T cells are thawed and cultured overnight in the presence of IL-2.Day 2: Enrichment, Staining and Antigen Stimulation of CD4+ and/or CD8+ T cells for 36-48 hours.Day3: Loading of T cells onto IsoCode chip.... More
Protocol
High-Throughput Detection of Degraded Polysorbate in Biological Formulations with FMM
Excipients like polysorbate are used in the majority of biologic products to improve stability. However, the polysorbate itself can degrade, impairing the stability of the API and contributing an additional aggregate source that can elicit an immunogenic response. Traditionally, monitoring only poly... More
Protocol
Transfecting Edit-R™ Cas9 mRNA with Synthetic Guide RNA in Cultured Mammalian Cells
This is an abbreviated protocol for transfecting Edit-R™ Cas9 mRNA (Cat #CAS11195, #CAS11859, or #CAS11860) with synthetic guide RNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent (Cat #T-2010-xx). Synthetic guide RNA can be either synthetic single guide RNA, or synthetic c... More
Protocol
How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data
Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during product development. Typically, this analysis is conducted after enzymatic release and tagging of the glycans followed by fluorescence and MS detection. However, that approach lose... More
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Protocol
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
Protocol
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Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
Protocol
Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More