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GEN Protocols

Drug Discovery

Drug Discovery

Application

Benchling for Protein and Peptide Therapeutics R&D

Proteins and peptides make up a wide variety of therapeutic molecules ranging from enzymes to cytokines. These therapeutics have several biological functions and interact with various biological pathways. New classes of proteins and peptides continue to be of significant research interest, but there... More


Protocol

CRISPRmod dCas9-VPR or dCas9- SALL1-SDS3 mRNA and synthetic guide RNA transfection protocol

The following is a protocol for transfecting CRISPRmod dCas9-VPR or dCas9-SALL1-SDS3 mRNA with synthetic guide RNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent (Cat #T-2010-xx). The protocol is written for transfection into 96-well tissue culture plates.... More


Protocol

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology

This new method takes advantage of Trapped Ion Mobility Spectrometry (TIMS), to further enhance MALDI-TDS by adding another dimension of separation in the gas phase.High confidence N- and C-terminal sequencing specifically are enhanced by combining high resolution (~ 60,000 RP) and 2 ppm mass accura... More


Protocol

Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography

We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno-associated virus (AAV) gene transfer vectors independent of their serotype and surface characteristics. SXC is performed by mixing an unpurified cell culture supernatant containing A... More


Protocol

Development and Validation of an Anion Exchange High-Performance Liquid Chromatography Method for Analysis of Empty Capsids and Capsids Encapsidating Genetic Material in a Purified Preparation of Recombinant Adeno-Associated Virus Serotype 5

The development of various manufacturing platforms and analytical technologies has substantially contributed to successfully translating the recombinant adeno-associated viral vector from the laboratory to the clinic. The active deployment of these analytical technologies for process and product cha... More


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