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GEN Protocols

Drug Discovery

Application

Benchling for Antibody R&D

Innovations in monoclonal antibody technology, bispecifics, and antibody-drug conjugates continue to push the envelope of antibody efficacy and safety. But the industry requires a new kind of digital solution that can offer a holistic view of antibody research and development, integrating large and ... More


Application

Benchling for Gene Therapy & Gene Editing R&D

Modern gene therapy requires investigation of a wide variety of genes of interest, engineering of delivery vectors, and optimization of vector production processes. This necessitates a strong digital foundation to comprehensively characterize gene therapy candidates to bring the best products into t... More


Application

The Benchling Life Sciences R&D Cloud

Life science R&D is becoming more complex. Companies need software built for the new way of doing science, or they risk falling behind the next generation of therapeutic development. Enter Benchling. Benchling connects the entire R&D lifecycle — from project documentation and data acquisition to seq... More


Protocol

Lentiviral titering by crystal violet staining

This protocol provides an example for titering lentiviral particles using crystal violet staining in a 6-well format to obtain transducing units per mL (TU/mL). Once lentiviral particles are packaged and collected, small aliquots should be frozen at –80°C. One aliquot should be thawed on ice and use... More


Protocol

Multiplexed, trackable CRISPR-based genome engineering for optimization of heterologous protein expression and activity in E. coli

This work demonstrates the use of the Onyx platform for interrogation and optimization of heterologous proteins in E. coli. Compared to traditional methods for protein engineering, such as random mutagenesis or targeted site-directed mutagenesis approaches, the Onyx technology dramatically increases... More