GEN Protocols

Drug Discovery

Protocol

CRISPRmod dCas9-VPR or dCas9- SALL1-SDS3 mRNA and synthetic guide RNA transfection protocol

The following is a protocol for transfecting CRISPRmod dCas9-VPR or dCas9-SALL1-SDS3 mRNA with synthetic guide RNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent (Cat #T-2010-xx). The protocol is written for transfection into 96-well tissue culture plates.... More


Protocol

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology

This new method takes advantage of Trapped Ion Mobility Spectrometry (TIMS), to further enhance MALDI-TDS by adding another dimension of separation in the gas phase.High confidence N- and C-terminal sequencing specifically are enhanced by combining high resolution (~ 60,000 RP) and 2 ppm mass accura... More


Protocol

Generating and maintaining uniform 3D spheroids in long term cultures

This instruction explains the basic operating procedure for generating and maintaining longterm three-dimensional spheroid cultures on PrimeSurface® 96 Slit-well plates. Instructions recommended here are the best operating protocols but depending upon your cell line and assay, some optimizations may... More


Application

Benchling & Jounce Therapeutics: Migrating to an All-in-One Cloud Solution Saves Time on Data Management

Jounce Therapeutics uses a number of different analytical techniques to identify biomarkers in the tumor microenvironment (TME) that can help them match their drug candidates with the right patients. But they needed a way to manage all their assay and antibody data from concept to IND across differe... More


Application

Benchling & uniQure: Bringing a gene therapy pioneer from paper to the cloud

uniQure is rapidly advancing a pipeline of AAV-based gene therapies to treat patients with hemophilia, Huntington’s disease, and other severe genetic diseases, but they needed a digital solution that could track their gene therapy workflows end-to-end and enable international collaboration. Learn ho... More