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GEN Protocols

Cancer Research

Cancer Research

Protocol

School Nurse Perspectives on Do-It-Yourself Automated Pancreas Systems in the School Setting

Do-it-yourself (DIY) artificial pancreas systems (APSs) are gaining popularity among children with type 1 diabetes. Little is known about how school systems provide care for children who use DIY APSs, and available guidance for schools is limited. This study explored school staff perspectives on DIY... More


Protocol

Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, e... More


Protocol

Long Term Storage, Recycling and Simplified Calcium Phosphate Transfection Protocols for Cell Cultures

Long term storage of cells is vital for use in the future experiments. Current methods of storing cells under liquid nitrogen vapor is costly, and requires regular monitoring and filling of the storage tank. We stored several cell lines in -80oC freezer over a decade and revived them successfully. S... More


Application

Recombinant RabMAb®: Reliable antibodies for reproducible results

Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More


Application

Antibody conjugation guide

Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More


Application

Batch-to-batch consistency of premium grade bioactive proteins

​​​​This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ​​​​​​​​​​​​​​​​​​​​... More


Application

Technical tips for ELISA and multiplex immunoassay development

Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More


Video

GEN Protocols Expert Exchanges: Patient-Derived Pancreatic Cancer Organoids Predict Response to Therapy

Pancreatic cancer accounts forthree percentof all cancers in the United States and is responsible for seven percent of all cancer deaths. Miniature organoids cloned from pancreatic tumors in patients are being grown in laboratories to conduct assays that can predict the effectiveness of chemotherapy... More


Mixer

Cell Therapy: Autologous vs Allogeneic Cell Expansion

This Mixer discusses culture systems, integrated consumables, and technical considerations for cell expansion processes in the production of autologous and allogeneic cell therapy products. Our expert panelists will discuss the implementation of scalable, cost-efficient technologies and process opti... More


Primer

Planning Your Next Chemogenetic Experiment

Chemogenetic experiments have been used to study a wide variety of behaviors, such as triggers for substance abuse,eating disorders, obesity, obesity-associated metabolic abnormalities, long term memory, hypertension, locomotor ability, as well as pain, startle, and fear responses. For instance,Hori... More


Protocol

MALDI Guided SpatialOMx uncovers proteomic profiles in tumor subpopulations of breast cancer

The timsTOF fleX system bridges a current gap by providing MALDI Imaging and in-depth proteomics analysis in just one instrument. The instrument offers all benefits of a timsTOF Pro for timeefficient and sensitive proteomics, combined with a high-resolution MALDI source and stage. Using PASEF techno... More


Protocol

CodePlex Secretome

This protocol outlines the standard method for thawing and loading samples onto the CodePlex chip. ... More


Protocol

IsoCode Single-Cell Adaptive Immune: Human CAR-T Protocol Using AF647-Conjugated Antibodies to CD4 and CD8

Overview of ProtocolDay 1: Cryopreserved CAR-T cells are thawed and cultured overnight in the presence of IL-2. Cryopreserved target cell lines are thawed, cultured, and passaged several days prior to beginning the experiment.Day 2: Enrichment and Antigen-Stimulation of CD8 + and/or CD4 + CAR-T cell... More


Protocol

IsoCode Single-Cell Adaptive Immune: Human Bispecific Protocol

Overview of ProtocolDay 1: Cryopreserved T cells are thawed and cultured overnight in the presence of IL-2.Day 2: Enrichment, Staining and Antigen Stimulation of CD4+ and/or CD8+ T cells for 36-48 hours.Day3: Loading of T cells onto IsoCode chip.... More


Protocol

Single-Cell Intracellular Proteome: HeLa Cell Protocol

Overview of Protocol:Day 1-3: Cryopreserved HeLa cells are thawed and cultured for 72 hours.Day 4: Staining and stimulating of HeLa cells followed by immediate loading onto IsoCode Chip... More


Protocol

AAV Aggregate Quantitation and Identification with the Aura System

Adeno-associated viruses (AAVs) have made the promise of gene therapy a reality by enabling safe, targeted delivery of curative genetic payloads. The formation of aggregated viral vector is an important critical quality attribute to measure because they lead to adverse immunogenic events and are an ... More


Protocol

Detect Specific Protein Aggregates in Biologic Formulations with the Aura Immunoassay

Biologic co-formulations target multiple pharmacologically active sites to increase therapy efficacy. However, either biologic can be a source of protein aggregation that can negatively influence the product’s safety, efficacy, and therapy. Aura systems are the only particle analyzer that can pinpoi... More


Protocol

Evaluate Cell Therapy Product Purity with Aura CL

The CAR-T development workflow is a multi-step process that includes transfection of T cells with a viral vector to express a chimeric antigen and activation/expansion of the CAR-T using a molecule like a Dynabead conjugated to CD3/CD28. Each of these steps is a potential source of contamination tha... More


Protocol

IsoCode Single-Cell Adaptive Immune: TCR-T Protocol

A. Overview of ProtocolDay 1: Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2. Cryopreserved target cell lines are thawed, cultured and passaged several days prior to beginning the experiment.Day 2: Enrichment, Staining, and Antigen Stimulation of CD4+ and/or CD8+... More


Protocol

IsoCode Single-Cell Innate Immune: Human Monocyte Protocol Using Membrane Stain

A. OverviewOverview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in complete RPMI media.Day 2: Enrichment and Stimulation of Monocytes for 24 hours.Day 3: Staining and Loading of Monocytes onto IsoCode Chip.... More


Application

Recombinant RabMAb®: Reliable antibodies for reproducible results

Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More


Application

Antibody conjugation guide

Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More


Application

Batch-to-batch consistency of premium grade bioactive proteins

​​​​This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ​​​​​​​​​​​​​​​​​​​​... More


Application

Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology

Precedent Protocols and Processes ensure that enrichment, stimulation and labeling will result in maximizing the correlative data achieved, while minimizing non-specific impact on the cells themselves. Highlights: • Published enrichment, stimulation, and staining protocols throughout immunotherapy ... More


Application

Technical tips for ELISA and multiplex immunoassay development

Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More


Primer

Planning Your Next Chemogenetic Experiment

Chemogenetic experiments have been used to study a wide variety of behaviors, such as triggers for substance abuse,eating disorders, obesity, obesity-associated metabolic abnormalities, long term memory, hypertension, locomotor ability, as well as pain, startle, and fear responses. For instance,Hori... More


Mixer

Cell Therapy: Autologous vs Allogeneic Cell Expansion

This Mixer discusses culture systems, integrated consumables, and technical considerations for cell expansion processes in the production of autologous and allogeneic cell therapy products. Our expert panelists will discuss the implementation of scalable, cost-efficient technologies and process opti... More


Video

GEN Protocols Expert Exchanges: Patient-Derived Pancreatic Cancer Organoids Predict Response to Therapy

Pancreatic cancer accounts forthree percentof all cancers in the United States and is responsible for seven percent of all cancer deaths. Miniature organoids cloned from pancreatic tumors in patients are being grown in laboratories to conduct assays that can predict the effectiveness of chemotherapy... More


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