Cancer Research
Cancer Research
Protocol
Detect Specific Protein Aggregates in Biologic Formulations with the Aura Immunoassay
Biologic co-formulations target multiple pharmacologically active sites to increase therapy efficacy. However, either biologic can be a source of protein aggregation that can negatively influence the product’s safety, efficacy, and therapy. Aura systems are the only particle analyzer that can pinpoi... More
Protocol
Evaluate Cell Therapy Product Purity with Aura CL
The CAR-T development workflow is a multi-step process that includes transfection of T cells with a viral vector to express a chimeric antigen and activation/expansion of the CAR-T using a molecule like a Dynabead conjugated to CD3/CD28. Each of these steps is a potential source of contamination tha... More
Protocol
IsoCode Single-Cell Adaptive Immune: TCR-T Protocol
A. Overview of ProtocolDay 1: Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2. Cryopreserved target cell lines are thawed, cultured and passaged several days prior to beginning the experiment.Day 2: Enrichment, Staining, and Antigen Stimulation of CD4+ and/or CD8+... More
Protocol
IsoCode Single-Cell Innate Immune: Human Monocyte Protocol Using Membrane Stain
A. OverviewOverview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in complete RPMI media.Day 2: Enrichment and Stimulation of Monocytes for 24 hours.Day 3: Staining and Loading of Monocytes onto IsoCode Chip.... More
Protocol
IsoCode Single-Cell Innate Immune: Human CD34+ Myeloid Stem Cell Protocol
A. Overview1. Overview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.Day 2: Enrichment and Stimulation of CD34+ cells for 24 hours.Day 3: Staining and Loading of CD34+ cells onto IsoCode chip.NOTE: This protocol outlines the standard method for thawi... More
Protocol
Isolation of Mononuclear Cell Fractions Using Your Sepax™ System
The purpose of this protocol is to provide instructions on how to isolate mononuclear cell fractions of apheresis product, bone marrow, or umbilical cord blood using the Sepax™ system with initial volumes of 30 to 120 mL and selectable final volumes of 8-20 mL or 45 mL. This protocol applies to anyo... More
Protocol
How to run cryopreservation protocols using a VIA Freeze™ system
The purpose of this protocol is to give the reader a comprehensive understanding of how to set up and operate the Cytiva VIA Freeze™ Uno, VIA Freeze™ Duo, and VIA Freeze™ Quad units.This protocol applies to anyone performing a procedure with the VIA Freeze™ units. Once training on this document is c... More
Protocol
Performing a manual run on your Xuri™ Cell Expansion System W25
The purpose of this protocol is to provide instructions on how to perform a manual run on the Xuri™ Cell Expansion System W25 using UNICORN™ software.This protocol applies to anyone performing a manual run on the Xuri™ Cell Expansion System W25.... More
Protocol
Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates
Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
IsoCode Single-Cell Innate Immune: Human CD34+ Myeloid Stem Cell Protocol
A. Overview1. Overview of ProtocolDay 1: Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.Day 2: Enrichment and Stimulation of CD34+ cells for 24 hours.Day 3: Staining and Loading of CD34+ cells onto IsoCode chip.NOTE: This protocol outlines the standard method for thawi... More
Protocol
Isolation of Mononuclear Cell Fractions Using Your Sepax™ System
The purpose of this protocol is to provide instructions on how to isolate mononuclear cell fractions of apheresis product, bone marrow, or umbilical cord blood using the Sepax™ system with initial volumes of 30 to 120 mL and selectable final volumes of 8-20 mL or 45 mL. This protocol applies to anyo... More
Protocol
How to run cryopreservation protocols using a VIA Freeze™ system
The purpose of this protocol is to give the reader a comprehensive understanding of how to set up and operate the Cytiva VIA Freeze™ Uno, VIA Freeze™ Duo, and VIA Freeze™ Quad units.This protocol applies to anyone performing a procedure with the VIA Freeze™ units. Once training on this document is c... More
Protocol
Performing a manual run on your Xuri™ Cell Expansion System W25
The purpose of this protocol is to provide instructions on how to perform a manual run on the Xuri™ Cell Expansion System W25 using UNICORN™ software.This protocol applies to anyone performing a manual run on the Xuri™ Cell Expansion System W25.... More
Protocol
Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates
Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More
Protocol
Detergent-Free Nuclei Isolation
- OMICS
- Drug Discovery
- Translational Medicine
- Computational Biosciences
- Cancer Research
- Genome Editing
A novel spin column-based approach to isolate intact single nuclei from tissues (fresh or frozen) and cells without using a detergent. The protocol is fast, easy, and reliable, producing high-quality single nuclei with minimized clumping and possible leakage of the nuclear envelope. The protocol is ... More
Protocol
School Nurse Perspectives on Do-It-Yourself Automated Pancreas Systems in the School Setting
Do-it-yourself (DIY) artificial pancreas systems (APSs) are gaining popularity among children with type 1 diabetes. Little is known about how school systems provide care for children who use DIY APSs, and available guidance for schools is limited. This study explored school staff perspectives on DIY... More
Protocol
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions
Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, e... More
Protocol
Long Term Storage, Recycling and Simplified Calcium Phosphate Transfection Protocols for Cell Cultures
Long term storage of cells is vital for use in the future experiments. Current methods of storing cells under liquid nitrogen vapor is costly, and requires regular monitoring and filling of the storage tank. We stored several cell lines in -80oC freezer over a decade and revived them successfully. S... More
Application
Recombinant RabMAb®: Reliable antibodies for reproducible results
Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More
Application
Antibody conjugation guide
Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More
Application
Batch-to-batch consistency of premium grade bioactive proteins
This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ... More
Application
Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology
Precedent Protocols and Processes ensure that enrichment, stimulation and labeling will result in maximizing the correlative data achieved, while minimizing non-specific impact on the cells themselves. Highlights: • Published enrichment, stimulation, and staining protocols throughout immunotherapy ... More
Application
Technical tips for ELISA and multiplex immunoassay development
Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More
Primer
Planning Your Next Chemogenetic Experiment
Chemogenetic experiments have been used to study a wide variety of behaviors, such as triggers for substance abuse,eating disorders, obesity, obesity-associated metabolic abnormalities, long term memory, hypertension, locomotor ability, as well as pain, startle, and fear responses. For instance,Hori... More
Mixer
Cell Therapy: Autologous vs Allogeneic Cell Expansion
This Mixer discusses culture systems, integrated consumables, and technical considerations for cell expansion processes in the production of autologous and allogeneic cell therapy products. Our expert panelists will discuss the implementation of scalable, cost-efficient technologies and process opti... More
Video
GEN Protocols Expert Exchanges: Patient-Derived Pancreatic Cancer Organoids Predict Response to Therapy
Pancreatic cancer accounts forthree percentof all cancers in the United States and is responsible for seven percent of all cancer deaths. Miniature organoids cloned from pancreatic tumors in patients are being grown in laboratories to conduct assays that can predict the effectiveness of chemotherapy... More