Analytical Testing
Analytical Testing
Protocol
Preparing a Brain-Mimetic 3D Matrix for Culturing Human Pluripotent Stem Cells
This is a protocol for culturing human pluripotent stem cells (hPSCs) using a 3D matrix. It provides instructions for preparing a brain-mimetic 3D scaffold that can support neuronal growth and for culturing hPSCs. This injectable scaffold can be potentially employed in the field of regenerative medi... More
Protocol
Radiolabelling Nucleic Acids with [γ-32P] ATP for Analytical Assays
Nucleic acids are readily modified with tags that enable detection, purification, or tracking their interactions with other molecules. One of the standard labels used to generate nucleic acid probes includes radioactive phosphates because they allow for sensitive and rapid detection. This protocol d... More
Protocol
USP Compendial Particle Measurements with the Aura System
The Aura platform provides a rapid and robust USP 788 compendial method for subvisible particle analysis, a critical quality attribute. It can handle a wide range of sample types and can analyze anywhere from 5 µL to 10 mL of sample, enabling continuity of method from developability assessment to lo... More
Protocol
USP Particle Count Standards with Aura and Particle Vue 3.1
All membrane microscopy methods must generate accurate and reproducible counts for bead counting standards. We present a validated methodology for accurately counting United States Pharmacopeia (USP) microsphere count standards on Aura systems by seamlessly calculating the cumulative area of all the... More
Protocol
Evaluate Cell Therapy Product Purity with Aura CL
The CAR-T development workflow is a multi-step process that includes transfection of T cells with a viral vector to express a chimeric antigen and activation/expansion of the CAR-T using a molecule like a Dynabead conjugated to CD3/CD28. Each of these steps is a potential source of contamination tha... More
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Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
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An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish -
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A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration -
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Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function -
Protocol
Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
Protocol
Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More