Analytical Testing
Protocol
An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish
The study of swimming behavior is an important part of fish biology research and the swim tunnel is used to study swimming performance as well as metabolism of fish. In this investigation, we have developed a user-friendly, automated, modular, and low-cost swim tunnel that permits to study the perfo... More
Protocol
A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration
Osteochondral tissue repair represents a common clinical need, with multiple approaches in tissue engineering and regenerative medicine being investigated for the repair of defects of articular cartilage and subchondral bone. A full thickness rabbit femoral condyle defect is a clinically relevant mo... More
Protocol
Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function
The surface of articular cartilage is integral to smooth, low-friction joint articulation. However, the majority of cartilage literature rarely includes measurements of surface characteristics and function. This may, in part, be due to a shortage of or unfamiliarity with fast, nondestructive, and, p... More
Protocol
Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors
Adeno-associated virus (AAV) is currently the most popular gene delivery vector for in vivo gene therapy. However, variability in titration methods between different laboratories affects the reproducibility of experiments and evaluation of safety and efficacy in clinical trials. We describe an optim... More
Protocol
Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography
We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno-associated virus (AAV) gene transfer vectors independent of their serotype and surface characteristics. SXC is performed by mixing an unpurified cell culture supernatant containing A... More
The Latest
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Protocol
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions -
Protocol
An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish -
Protocol
A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration -
Protocol
Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function -
Protocol
Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors -
Protocol
Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography - More