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Application

Benchling for CRISPR Screening

CRISPR screening is a revolutionary methodology to identify genes of interest underlying a specific phenotype. CRISPR can be scaled up for screening applications because of the versatility of CRISPR/Cas9 in disrupting target DNA and the ease of gRNA/plasmid generation. However, large-scale use of th... More


Application

Benchling for Industrial Biotech R&D

Industrial biotechnology is transforming traditional manufacturing technologies by providing more eco-friendly approaches like bioprocessing and biomanufacturing. However, there are several hurdles for industrial biotech companies seeking to develop next-gen strains, novel processes, and new applica... More


Application

Benchling for Industrial Biotech R&D

Industrial biotechnology is transforming traditional manufacturing technologies by providing more eco-friendly approaches like bioprocessing and biomanufacturing. However, there are several hurdles for industrial biotech companies seeking to develop next-gen strains, novel processes, and new applica... More


Primer

Pathogen Detection Assays: Advantages, Disadvantages and Alternatives

Viral infections are difficult to diagnose based on the patient’s clinical presentation alone. A reliable and accurate diagnosis requires laboratory-based testing for the pathogen of interest. Such laboratory testing involves either the direct detection of viral antigens, or detection of virus-speci... More


Protocol

Radiolabelling Nucleic Acids with [γ-32P] ATP for Analytical Assays

Nucleic acids are readily modified with tags that enable detection, purification, or tracking their interactions with other molecules. One of the standard labels used to generate nucleic acid probes includes radioactive phosphates because they allow for sensitive and rapid detection. This protocol d... More


Primer

Assessing qRT-PCR COVID-19 Testing Results: What Can Go Wrong?

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes--an RNA-dependent DNA polymerase, also known as a reverse transcriptase, to copy the SARS-CoV-2 RNA into complement... More


Protocol

Generating and maintaining uniform 3D spheroids in long term cultures

This instruction explains the basic operating procedure for generating and maintaining longterm three-dimensional spheroid cultures on PrimeSurface® 96 Slit-well plates. Instructions recommended here are the best operating protocols but depending upon your cell line and assay, some optimizations may... More


Protocol

Streamlined High Throughput Drug Screening with PrimeSurface® White 384 well 3D plates

Using PrimeSurface® white plate, cancer spheroid cells can be formed and tested for drug screening using luminescence viability assay in the same well without the need for reagent transfer. This streamlined process shortens the experimental steps and time required in drug testing thus providing a ro... More


Protocol

Performing a manual run on your Xuri™ Cell Expansion System W25

The purpose of this protocol is to provide instructions on how to perform a manual run on the Xuri™ Cell Expansion System W25 using UNICORN™ software.This protocol applies to anyone performing a manual run on the Xuri™ Cell Expansion System W25.... More


Protocol

How to run cryopreservation protocols using a VIA Freeze™ system

The purpose of this protocol is to give the reader a comprehensive understanding of how to set up and operate the Cytiva VIA Freeze™ Uno, VIA Freeze™ Duo, and VIA Freeze™ Quad units.This protocol applies to anyone performing a procedure with the VIA Freeze™ units. Once training on this document is c... More


Protocol

Long Term Storage, Recycling and Simplified Calcium Phosphate Transfection Protocols for Cell Cultures

Long term storage of cells is vital for use in the future experiments. Current methods of storing cells under liquid nitrogen vapor is costly, and requires regular monitoring and filling of the storage tank. We stored several cell lines in -80oC freezer over a decade and revived them successfully. S... More


Protocol

Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, e... More


Protocol

An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish

The study of swimming behavior is an important part of fish biology research and the swim tunnel is used to study swimming performance as well as metabolism of fish. In this investigation, we have developed a user-friendly, automated, modular, and low-cost swim tunnel that permits to study the perfo... More


Protocol

A Rabbit Femoral Condyle Defect Model for Assessment of Osteochondral Tissue Regeneration

Osteochondral tissue repair represents a common clinical need, with multiple approaches in tissue engineering and regenerative medicine being investigated for the repair of defects of articular cartilage and subchondral bone. A full thickness rabbit femoral condyle defect is a clinically relevant mo... More


Protocol

Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function

The surface of articular cartilage is integral to smooth, low-friction joint articulation. However, the majority of cartilage literature rarely includes measurements of surface characteristics and function. This may, in part, be due to a shortage of or unfamiliarity with fast, nondestructive, and, p... More


Protocol

Optimized Protocol for Accurate Titration of Adeno-Associated Virus Vectors

Adeno-associated virus (AAV) is currently the most popular gene delivery vector for in vivo gene therapy. However, variability in titration methods between different laboratories affects the reproducibility of experiments and evaluation of safety and efficacy in clinical trials. We describe an optim... More


Protocol

Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography

We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno-associated virus (AAV) gene transfer vectors independent of their serotype and surface characteristics. SXC is performed by mixing an unpurified cell culture supernatant containing A... More


Protocol

Development and Validation of an Anion Exchange High-Performance Liquid Chromatography Method for Analysis of Empty Capsids and Capsids Encapsidating Genetic Material in a Purified Preparation of Recombinant Adeno-Associated Virus Serotype 5

The development of various manufacturing platforms and analytical technologies has substantially contributed to successfully translating the recombinant adeno-associated viral vector from the laboratory to the clinic. The active deployment of these analytical technologies for process and product cha... More


Protocol

Predictive Low-Glucose Suspend Necessitates Less Carbohydrate Supplementation to Rescue Hypoglycemia: Need to Revisit Current Hypoglycemia Treatment Guidelines

Current guidelines recommend 15–20 g of carbohydrate (CHO) for treatment of mild to moderate hypoglycemia. However, these guidelines do not account for reduced insulin during suspensions with predictive low-glucose suspend (PLGS). We assessed insulin suspensions, hypoglycemic events, and CHO treatme... More


Protocol

Insulin Metrics: Need for Development of Consensus Standards for Reporting of Insulin Dosing Data

The advent of connected insulin pens will generate an avalanche of digital insulin data, especially in the context of prandial- and multiple daily injection-insulin regimens. There is a need for the diabetes community to develop standards for such data, analogous to what has been achieved using the ... More


Application

Knock-out cell lines and their use in signaling pathways

Knock-out (KO) cell lines are powerful tools to study disease mechanisms, such as the effect of gene disruption on downstream pathways. In this application note, we highlight the effectiveness of Abcam KO cell lines that can be used to investigate signaling pathways, in this case, the TRAIL pathway.... More


Application

Knock-out cell lines and their use in signaling pathways

Knock-out (KO) cell lines are powerful tools to study disease mechanisms, such as the effect of gene disruption on downstream pathways. In this application note, we highlight the effectiveness of Abcam KO cell lines that can be used to investigate signaling pathways, in this case, the TRAIL pathway.... More


Application

Recombinant RabMAb®: Reliable antibodies for reproducible results

Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More


Application

Recombinant RabMAb®: Reliable antibodies for reproducible results

Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More


Application

Antibody conjugation guide

Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More


Application

Antibody conjugation guide

Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More


Application

Batch-to-batch consistency of premium grade bioactive proteins

​​​​This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ​​​​​​​​​​​​​​​​​​​​... More


Application

Batch-to-batch consistency of premium grade bioactive proteins

​​​​This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ​​​​​​​​​​​​​​​​​​​​... More


Application

Technical tips for ELISA and multiplex immunoassay development

Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More


Application

Technical tips for ELISA and multiplex immunoassay development

Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More


Primer

AI-Powered Phenotypic Profiling Revolutionizes Drug Discovery

Today, nine out of ten drugs fail in clinical trials. Furthermore, it takes over a decade and an average cost of $2 billion to develop and approve each medicine.1 An important underlying reason is the gap that exists between cell-based in vitro research and clinical research—often referred to as the... More


Primer

Bio-Electrospraying and Cell Electrospinning: A Sea of Applications

Electrospraying and electrospinning have been used for a wide range of applications ranging from coatings, propulsion, and pesticide sprays to scaffolding a wide range of advanced materials for applications in filtration and tissue engineering. Although electrospun scaffolds have been explored as ti... More


Primer

Quick Antibiotic Susceptibility Test for High-Threat Pathogens in Whole Blood Samples

By spreading so rapidly and wreaking so much havoc, COVID-19 has revealed the susceptibility of global societies to both natural pathogens and intentionally released bioterror agents. The latter include bacteria that have been engineered for enhanced antibiotic resistance.1 Once pathogenic bacteria ... More


Primer

Impedance Spectroscopy Measures Antimicrobial Susceptibility in Real Time

Antimicrobial drugs save millions of lives each year. However, in 2019, antibiotic-resistant microorganisms were associated with nearly 5 million deaths worldwide1. The alarming trend of bacterial antimicrobial resistance may further worsen as many COVID-19 patients are administered broad spectrum a... More


Primer

Methods to Improve Efficiency of Antimicrobial Susceptibility Testing: Microfluidic Impedance Cytometry

The global issue of antimicrobial resistance has been called a “slow motion catastrophe” resulting from the excessive and often inappropriate use of antibiotics to fight bacterial infections.1 A long-standing unmet need is the availability of a quick and easy clinical test to support a more evidence... More


Primer

Tips for Increasing Transfection Efficiency

Chemical transfection, which relies on the formation of a condensed complex of positively chargedliposomal or non-liposomal reagents and negatively charged nucleic acids,is the most common method for delivering foreign genetic material into cultured cells. Complexes are attracted to the negatively c... More


Primer

Selecting a Functional Oligonucleotide from a Random Mix

SELEX (Systematic Evolution of Ligands by EXponential enrichment) was first described and used in 1990 to isolate a functional oligonucleotide from a random pool of differently-shaped DNA or RNA molecules.1-2SELEX-derived oligonucleotides (RNA or single-stranded DNA) known as aptamers, bind with hig... More


Primer

Planning Your Next Chemogenetic Experiment

Chemogenetic experiments have been used to study a wide variety of behaviors, such as triggers for substance abuse,eating disorders, obesity, obesity-associated metabolic abnormalities, long term memory, hypertension, locomotor ability, as well as pain, startle, and fear responses. For instance,Hori... More


Primer

Immunodiagnostics in the Post-Covid Era

The benefits of globalization have ushered in the reality of fast-spreading infectious diseases. This has made the quick development of preventive vaccines and immune therapies imperative. Spread of pathogens through rapid and constant travel is compounded by the multipronged challenges ranging from... More


Primer

Dried Blood Spot Testing: Advantages and Disadvantages

In vitro diagnostics, marketed directly to consumers without the involvement of a health care provider are called direct-to-consumer tests, or DTC, which are facilitated through easy-to-collect sample matrices such as dried blood spots (DBS), saliva, or swabs that allow the safe and stable transport... More


Mixer

Organoids for Airway and Alveolar Disease Modeling

In this Mixer, we will discuss the development of respiratory airway and alveolar organoids derived from human iPSCs and how these are used in modeling respiratory diseases such as cystic fibrosis. Airway organoid models can be used for testing the therapeutic efficacy of candidate drugs for respira... More


Mixer

Cell Therapy: Autologous vs Allogeneic Cell Expansion

This Mixer discusses culture systems, integrated consumables, and technical considerations for cell expansion processes in the production of autologous and allogeneic cell therapy products. Our expert panelists will discuss the implementation of scalable, cost-efficient technologies and process opti... More


Mixer

Mapping Tissue Carbohydrates Using Mass Spectrometry

This mixer will be relevant for all interested in methods used in glycoproteomics research that aims to understand the pivotal role of carbohydrate motifs that modify the proteome.Glycoproteomics, a dynamic area of frontier research, provides a systems biology approach to understanding the biologica... More


Mixer

Viral Titer Assays and Interpretations

In this Mixer we will discuss some common methods used to measure viral titers and considerations one should bear in mind when interpreting the results from different assays used to quantify virus particles.Along with all things virology, the recent pandemic has led to focused interest in the accura... More


Mixer

Cell Therapy Development: Tackling Analytic Challenges

Cell therapy products are an exciting new class of medicinal products that offer opportunities to treat and cure diseases that cannot be treated using conventional therapeutic options. The development and manufacturing of cell therapy products pose several challenges compared to other biologics. Yet... More


Video

GEN Protocols Expert Exchanges: Critical ALS Biomarkers of Neuroinflammation & Oxidative Stress

Biomarkers are essential tools for the development of therapeutics, clinical trial design and patient stratification. Biomarkers also help evaluate the efficacy of therapeutic intervention on disease progression or relapse. Yet, biomarker driven patient stratification, a hallmark in oncology trials,... More


Video

GEN Protocols Expert Exchanges: Patient-Derived Pancreatic Cancer Organoids Predict Response to Therapy

Pancreatic cancer accounts forthree percentof all cancers in the United States and is responsible for seven percent of all cancer deaths. Miniature organoids cloned from pancreatic tumors in patients are being grown in laboratories to conduct assays that can predict the effectiveness of chemotherapy... More


Video

GEN Protocols Expert Exchanges: COVID-19 Immunodiagnostic Test Identifies Antibody Source

Need another COVID-19 booster?A new immunodiagnostic test called PictArray helps you decide. The test tells you exactly where your antibodies to the virus are coming from - whether your antibodies are from vaccines or SARS-CoV-2 infections, or both. The test detects SARS-CoV-2 antigens--both spike p... More


Audio

GEN Protocols Expert Exchanges: Next Generation Techniques to Develop Genetic Medicines

The past decade has seen a shift in drug discovery to include targeting the root cause of genetic diseases: loss-of-function, gain-of-function, and change-of-function mutations in the genome. Although first generation genetic medicines like gene therapies heralded a new approach to treating previous... More


Video

GEN Protocols Expert Exchanges: Tools and Techniques to Develop Therapies for Neurological Disorders

The human brain is an exquisitely complex organ, consisting of nearly one hundred billion neurons, an equivalent number of non-neuronal cells and trillions of synapses. Understanding the factors that play a role in the development of the structure and function of the brain is an essential step to de... More


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