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GEN Protocols

The dynamic new hub for editorially curated, freely accessible scientific protocols, and technical tips & tricks in biotechnology

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AAV Aggregate Quantitation and Identification with the Aura System

Adeno-associated viruses (AAVs) have made the promise of gene therapy a reality by enabling safe, targeted delivery of curative genetic payloads. The formation of aggregated viral vector is an important critical quality attribute to measure because they lead to adverse immunogenic events and are an ... More


Protocol

Studying protein-drug complexes under native conditions in the low nM range using MRMS

Protein ligand complexes need to be preserved through the electrospray and detection process. This work describes high resolution native mass spectrometric measurements of the enzyme carbonic anhydrase II (Figure 1) and the non-covalent complex with the substrate acetazolamide (Figure 2) using a sci... More


Protocol

Skin Freezing Technique for Living Cell Bank

Cell banks are especially important sources of studies for which living cells are needed, as well as a repository of genetic diversity in natural populations. We propose a simple protocol for field research that can maintain viable skin cells for in vitro replication and production of fibroblast lin... More


Protocol

CodePlex Secretome

This protocol outlines the standard method for thawing and loading samples onto the CodePlex chip. ... More


Protocol

A Protocol for Qualifying DNA Variants Associated with Complex Diseases like Ehlers-Danlos syndrome.

A protocol for DNA variant qualification adds medical perspective to consensus grading based on impact of the DNA change on its encoded RNA/protein and its prevalence in normal people versus those with the implicated disease. This clinically familiar 1-4+ qualification can encourage physician accept... More


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