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Application
A Fast and High Precision Influenza Vaccine Potency Assay
Fast and accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly scaling each process step. Traditionally assays such as Single Radial Immunodiffusion (SRID) and ELISA have their limitations; SRID, though the ‘gold ... More
Application
Strategies for the Development of a High Throughput Octet® Bio-Layer Interferometry Method to Measure Pharmacokinetics of Monoclonal Antibodies in Preclinical Animal Models
Therapeutic antibodies are developed to treat various diseases, including cancers, immunological disorders, and infectious diseases. The safety and efficacy of these therapeutic molecules may be influenced by the way they interact with the body. Therefore, it is vital to understand how a body reacts... More
Protocol
Long Term Storage, Recycling and Simplified Calcium Phosphate Transfection Protocols for Cell Cultures
Long term storage of cells is vital for use in the future experiments. Current methods of storing cells under liquid nitrogen vapor is costly, and requires regular monitoring and filling of the storage tank. We stored several cell lines in -80oC freezer over a decade and revived them successfully. S... More
Protocol
Predictive Low-Glucose Suspend Necessitates Less Carbohydrate Supplementation to Rescue Hypoglycemia: Need to Revisit Current Hypoglycemia Treatment Guidelines
Current guidelines recommend 15–20 g of carbohydrate (CHO) for treatment of mild to moderate hypoglycemia. However, these guidelines do not account for reduced insulin during suspensions with predictive low-glucose suspend (PLGS). We assessed insulin suspensions, hypoglycemic events, and CHO treatme... More
Protocol
Preparing a Brain-Mimetic 3D Matrix for Culturing Human Pluripotent Stem Cells
This is a protocol for culturing human pluripotent stem cells (hPSCs) using a 3D matrix. It provides instructions for preparing a brain-mimetic 3D scaffold that can support neuronal growth and for culturing hPSCs. This injectable scaffold can be potentially employed in the field of regenerative medi... More
Application
Knock-out cell lines and their use in signaling pathways
Knock-out (KO) cell lines are powerful tools to study disease mechanisms, such as the effect of gene disruption on downstream pathways. In this application note, we highlight the effectiveness of Abcam KO cell lines that can be used to investigate signaling pathways, in this case, the TRAIL pathway.... More
Application
Recombinant RabMAb®: Reliable antibodies for reproducible results
Poorly characterized antibodies often fail to provide consistent, reproducible results, thereby wasting materials, researcher time, and money. Compared to traditional polyclonal and monoclonal antibodies, recombinant antibodies exhibit very little batch-to-batch variability, providing scientists wit... More
Application
Antibody conjugation guide
Antibody conjugation, also known as antibody labeling, is the process of chemically linking an antibody to a specific tag. There are many common methods used to conjugate antibodies, although they can have complex protocols and usually require a certain level of skill and expertise. In this guide, w... More
Application
Batch-to-batch consistency of premium grade bioactive proteins
This white paper demonstrates the batch-to-batch consistency of our premium grade bioactive proteins (growth factors and cytokines). Designed to meet the exacting demands of cell and gene therapy applications, our premium grade proteins are ideal for controlled cell culture. ... More
Application
Technical tips for ELISA and multiplex immunoassay development
Developing a high performance sandwich ELISA or multiplex immunoassay can be complicated, and high performance requires careful development and validation. To help, we have created this guide based on what we have learned during the development of over 500 matched antibody pairs, ELISA kits, and mul... More
Annual Guide
